What does pyrosequencing mean?
Definitions for pyrosequencing
py·rose·quenc·ing
This dictionary definitions page includes all the possible meanings, example usage and translations of the word pyrosequencing.
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Wiktionary
pyrosequencingnoun
A technique used to sequence DNA using chemiluminescent enzymatic reactions
Wikidata
Pyrosequencing
Pyrosequencing is a method of DNA sequencing based on the "sequencing by synthesis" principle. It differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release on nucleotide incorporation, rather than chain termination with dideoxynucleotides. The technique was developed by Mostafa Ronaghi and Pål Nyrén at the Royal Institute of Technology in Stockholm in 1996. The desired DNA sequence is able to be determined by light emitted upon incorporation of the next complementary nucleotide by the fact that only one out of four of the possible A/T/C/G nucleotides are added and available at a time so that only one letter can be incorporated on the single stranded template. The intensity of the light determines if there are more than one of these "letters" in a row. The previous nucleotide letter is degraded before the next nucleotide letter is added for synthesis: allowing for the possible revealing of the next nucleotide via the resulting intensity of light. This process is repeated with each of the four letters until the DNA sequence of the single stranded template is determined.
Numerology
Chaldean Numerology
The numerical value of pyrosequencing in Chaldean Numerology is: 1
Pythagorean Numerology
The numerical value of pyrosequencing in Pythagorean Numerology is: 8
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"pyrosequencing." Definitions.net. STANDS4 LLC, 2024. Web. 27 Apr. 2024. <https://www.definitions.net/definition/pyrosequencing>.
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