What does microscopy, phase-contrast mean?
Definitions for microscopy, phase-contrast
mi·croscopy, phase-con·trast
This dictionary definitions page includes all the possible meanings, example usage and translations of the word microscopy, phase-contrast.
Wikipedia
microscopy, phase-contrast
Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than a vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium. Changes in amplitude (brightness) arise from the scattering and absorption of light, which is often wavelength-dependent and may give rise to colors. Photographic equipment and the human eye are only sensitive to amplitude variations. Without special arrangements, phase changes are therefore invisible. Yet, phase changes often convey important information. Phase-contrast microscopy is particularly important in biology. It reveals many cellular structures that are invisible with a bright-field microscope, as exemplified in the figure. These structures were made visible to earlier microscopists by staining, but this required additional preparation and death of the cells. The phase-contrast microscope made it possible for biologists to study living cells and how they proliferate through cell division. It is one of the few methods available to quantify cellular structure and components that does not use fluorescence. After its invention in the early 1930s, phase-contrast microscopy proved to be such an advancement in microscopy that its inventor Frits Zernike was awarded the Nobel Prize in Physics in 1953.
U.S. National Library of Medicine
Microscopy, Phase-Contrast
A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate.
Numerology
Chaldean Numerology
The numerical value of microscopy, phase-contrast in Chaldean Numerology is: 9
Pythagorean Numerology
The numerical value of microscopy, phase-contrast in Pythagorean Numerology is: 7
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"microscopy, phase-contrast." Definitions.net. STANDS4 LLC, 2024. Web. 29 Apr. 2024. <https://www.definitions.net/definition/microscopy%2C+phase-contrast>.
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