What does heteroduplex analysis mean?
Definitions for heteroduplex analysis
het·erodu·plex ana·ly·sis
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Wikipedia
Heteroduplex analysis
Heteroduplex analysis (HDA) is a method in biochemistry used to detect point mutations in DNA since 1992. Heteroduplexes are dsDNA molecules that have one or more mismatched pairs, on the other hand homoduplexes are dsDNA which are perfectly paired. This method of analysis depend up on the fact that heteroduplexes shows reduced mobility relative to the homoduplex DNA. heteroduplexes are formed between different DNA alleles. In a mixture of wild-type and mutant amplified DNA, heteroduplexes are formed in mutant alleles and homoduplexes are formed in wild-type alleles. There are two types of heteroduplexes based on type and extent of mutation in the DNA. Small deletions or insertion create bulge-type heteroduplexes which is stable and is verified by electron microscope. Single base substitutions creates more unstable heteroduplexes called bubble-type heteroduplexes, because of low stability it is difficult to visualize in electron microscopy. HDA is widely used for rapid screening of mutation of the 3 bp p.F508del deletion in the CFTR gene.
U.S. National Library of Medicine
Heteroduplex Analysis
A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.
Numerology
Chaldean Numerology
The numerical value of heteroduplex analysis in Chaldean Numerology is: 5
Pythagorean Numerology
The numerical value of heteroduplex analysis in Pythagorean Numerology is: 1
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"heteroduplex analysis." Definitions.net. STANDS4 LLC, 2024. Web. 26 Apr. 2024. <https://www.definitions.net/definition/heteroduplex+analysis>.
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