Definitions for enzyme multiplied immunoassay technique
en·zyme mul·ti·plied im·munoas·say tech·nique

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1. Enzyme multiplied immunoassay technique

   Enzyme multiplied immunoassay technique (EMIT) is a common method for qualitative and quantitative determination of therapeutic and recreational drugs and certain proteins in serum and urine.It is an immunoassay in which a drug or metabolite in the sample competes with a drug/metabolite labelled with an enzyme, to bind to an antibody. The more drug there is in the sample, the more free enzyme there will be, and the increased enzyme activity causes a change in color.: 70 Determination of drug levels in serum is particularly important when the difference in the concentrations needed to produce a therapeutic effect and adverse side reactions (the therapeutic window) is small. EMIT therapeutic drug monitoring tests provide accurate information about the concentration of such drugs such as immunosuppressant drugs and some antibiotics.EMIT urine assays for drugs such as cannabinoids, morphine, and amphetamine are designed to detect the drug itself or a metabolite of the drug present in a concentration above a pre-specified minimum detection cutoff limit. In the U.S., the cutoff limits must be set in accordance with Mandatory Guidelines for Federal Workplace Drug Testing Programs that were developed by SAMHSA (The Substance Abuse and Mental Health Services Administration is a branch of the U.S. Department of Health and Human Services). The setting of reasonable cutoff limits help reduce false positive results that occur from assay limitations. Because of the social and legal consequences, a positive test result must be confirmed by an alternative method, usually Gas Chromatography/Mass spectrometry. As an example the SAMHSA cutoffs for cannabinoids are 50 ng/ml for the immunoassay and 15 ng/ml as confirmed by GC/MS. Immunoassays that do not conform with SAMHSA, featuring a cutoff of 20 ng/ml, have been shown to produce false positives from passive inhalation of marijuana smoke.

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1. Enzyme multiplied immunoassay technique

   Enzyme multiplied immunoassay technique is a common method for qualitative and quantitative determination of drugs and certain proteins in serum and urine. First introduced by Syva Company in 1973, it is the first "homogeneous immunoassay" to be widely used commercially. Homogeneous immunoassays can be carried out using exceptionally simple and rapid mix and read protocols. The most widely used applications for EMIT are for therapeutic drug monitoring and as a primary screen for abused drugs and their metabolites in. The original US patents covering major aspects of the method, 3,817,837 and 3,875,011, have expired although US patent 6,455,288 that covers a major improvement is still valid. While still sold by Siemens Healthcare under its original trade name, EMIT, assay kits with different names that employ the same technology are supplied by other companies. Determination of drug levels in serum is particularly important when the difference in the concentrations needed to produce a therapeutic effect and adverse side reactions is small, the therapeutic window. EMIT therapeutic drug monitoring tests provide accurate information about the concentration of such drugs such as digoxin and other cardioactive drugs, theophylline, anti-epileptic drugs, and antibiotics. EMIT urine assays for abused drugs such as cannabinoids, morphine, and amphetamine are designed to detect the drug itself or a metabolite of the drug present in a concentration above a pre-specified minimum detection limit. The assays are highly accurate but, like all drug abuse assays, they are susceptible to attempts by the patient to circumvent the test by adulteration of the sample. Because of the social and legal consequences, a positive test result should be confirmed by an alternative method, usually Mass spectrometry.

U.S. National Library of MedicineRate this definition:0.0 / 0 votes

1. Enzyme Multiplied Immunoassay Technique

   An immunoenzyme test for the presence of drugs and other substances in urine and blood. The test uses enzyme linked antibodies that react only with the particular drug for which the sample is being tested.

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Numerology

1. Chaldean Numerology

   The numerical value of enzyme multiplied immunoassay technique in Chaldean Numerology is: 2

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References

1. ^ Wikipedia
   https://en.wikipedia.org/wiki/Enzyme_Multiplied_Immunoassay_Technique
2. ^ Wikidata
   https://www.wikidata.org/w/index.php?search=enzyme multiplied immunoassay technique