Definitions for ribonuclease t1
This page provides all possible meanings and translations of the word ribonuclease t1
Ribonuclease T1 is a fungal endonuclease that cleaves single-stranded RNA after guanine residues, i.e., on their 3' end; the most commonly studied form of this enzyme is the version found in the mold Aspergillus oryzae. Owing to its specificity for guanine, RNase T1 is often used to digest denatured RNA prior to sequencing. Similar to other ribonucleases such as barnase and RNase A, ribonuclease T1 has been popular for folding studies. Structurally, ribonuclease T1 is a small α+β protein with a four-stranded, antiparallel beta sheet covering a long alpha helix. RNase T1 has two disulfide bonds, Cys2-Cys10 and Cys6-Cys103, of which the latter contributes more to its folding stability; complete reduction of both disulfides usually unfolds the protein, although its folding can be rescued with high salt concentrations.
U.S. National Library of Medicine
An enzyme catalyzing the endonucleolytic cleavage of RNA at the 3'-position of a guanylate residue. EC 22.214.171.124.
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