What does fluorescein isothiocyanate mean?
Definitions for fluorescein isothiocyanate
flu·o·res·cein isoth·io·cyanate
This dictionary definitions page includes all the possible meanings, example usage and translations of the word fluorescein isothiocyanate.
Princeton's WordNet
fluorescein isothiocyanate, fluorescein isocyanatenoun
a fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence
ChatGPT
fluorescein isothiocyanate
Fluorescein isothiocyanate (FITC) is a chemical compound commonly used as a fluorescent tracer in biological research, including fluorescence microscopy and flow cytometry. It has the ability to bind to proteins, making them visible under a fluorescent microscope. This allows scientists to track various processes and structures within cells and other biological systems. FITC fluoresces green when excited by light of a specific wavelength and is often used in immunostaining. Its chemical formula is C21H11NO5S.
Wikidata
Fluorescein isothiocyanate
Fluorescein isothiocyanate is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group, replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. A succinimidyl-ester functional group attached to the fluorescein core, creating NHS-fluorescein, forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.
Matched Categories
Numerology
Chaldean Numerology
The numerical value of fluorescein isothiocyanate in Chaldean Numerology is: 6
Pythagorean Numerology
The numerical value of fluorescein isothiocyanate in Pythagorean Numerology is: 3
Translation
Find a translation for the fluorescein isothiocyanate definition in other languages:
Select another language:
- - Select -
- 简体中文 (Chinese - Simplified)
- 繁體中文 (Chinese - Traditional)
- Español (Spanish)
- Esperanto (Esperanto)
- 日本語 (Japanese)
- Português (Portuguese)
- Deutsch (German)
- العربية (Arabic)
- Français (French)
- Русский (Russian)
- ಕನ್ನಡ (Kannada)
- 한국어 (Korean)
- עברית (Hebrew)
- Gaeilge (Irish)
- Українська (Ukrainian)
- اردو (Urdu)
- Magyar (Hungarian)
- मानक हिन्दी (Hindi)
- Indonesia (Indonesian)
- Italiano (Italian)
- தமிழ் (Tamil)
- Türkçe (Turkish)
- తెలుగు (Telugu)
- ภาษาไทย (Thai)
- Tiếng Việt (Vietnamese)
- Čeština (Czech)
- Polski (Polish)
- Bahasa Indonesia (Indonesian)
- Românește (Romanian)
- Nederlands (Dutch)
- Ελληνικά (Greek)
- Latinum (Latin)
- Svenska (Swedish)
- Dansk (Danish)
- Suomi (Finnish)
- فارسی (Persian)
- ייִדיש (Yiddish)
- հայերեն (Armenian)
- Norsk (Norwegian)
- English (English)
Word of the Day
Would you like us to send you a FREE new word definition delivered to your inbox daily?
Citation
Use the citation below to add this definition to your bibliography:
Style:MLAChicagoAPA
"fluorescein isothiocyanate." Definitions.net. STANDS4 LLC, 2024. Web. 28 Apr. 2024. <https://www.definitions.net/definition/fluorescein+isothiocyanate>.
Discuss these fluorescein isothiocyanate definitions with the community:
Report Comment
We're doing our best to make sure our content is useful, accurate and safe.
If by any chance you spot an inappropriate comment while navigating through our website please use this form to let us know, and we'll take care of it shortly.
Attachment
You need to be logged in to favorite.
Log In