What does electroporation mean?
Definitions for electroporation
elec·tro·po·ra·tion
This dictionary definitions page includes all the possible meanings, example usage and translations of the word electroporation.
Wiktionary
electroporationnoun
the creation of transient pores in the cell membrane, through the application of a high-voltage electrical pulse
Wikidata
Electroporation
Electroporation, or electropermeabilization, is a significant increase in the electrical conductivity and permeability of the cell plasma membrane caused by an externally applied electrical field. It is usually used in molecular biology as a way of introducing some substance into a cell, such as loading it with a molecular probe, a drug that can change the cell's function, or a piece of coding DNA. Electroporation is a dynamic phenomenon that depends on the local transmembrane voltage at each point on the cell membrane. It is generally accepted that for a given pulse duration and shape, a specific transmembrane voltage threshold exists for the manifestation of the electroporation phenomenon. This leads to the definition of an electric field magnitude threshold for electroporation. That is, only the cells within areas where E≧Eth are electroporated. If a second threshold is reached or surpassed, electroporation will compromise the viability of the cells, i.e., irreversible electroporation. In molecular biology, the process of electroporation is often used for the transformation of bacteria, yeast, and plant protoplasts. In addition to the lipid membranes, bacteria also have cell walls which are different from the lipid membranes and are made of peptidoglycan and its derivatives. However, the walls are naturally porous and only act as stiff shells that protect bacteria from severe environmental impacts. If bacteria and plasmids are mixed together, the plasmids can be transferred into the cell after electroporation. Several hundred volts across a distance of several millimeters are typically used in this process. Afterwards, the cells have to be handled carefully until they have had a chance to divide producing new cells that contain reproduced plasmids. This process is approximately ten times as effective as chemical transformation.
U.S. National Library of Medicine
Electroporation
A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.
Numerology
Chaldean Numerology
The numerical value of electroporation in Chaldean Numerology is: 1
Pythagorean Numerology
The numerical value of electroporation in Pythagorean Numerology is: 6
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"electroporation." Definitions.net. STANDS4 LLC, 2024. Web. 25 Apr. 2024. <https://www.definitions.net/definition/electroporation>.
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