What does diethylpyrocarbonate mean?
Definitions for diethylpyrocarbonate
di·ethylpy·ro·car·bon·ate
This dictionary definitions page includes all the possible meanings, example usage and translations of the word diethylpyrocarbonate.
Wiktionary
diethylpyrocarbonatenoun
The diethyl ester of pyrocarbonic acid; it is used in biochemistry to inactivate ribonucleases
Wikidata
Diethylpyrocarbonate
Diethylpyrocarbonate, also called diethyl dicarbonate, is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils. It does so by the covalent modification of histidine, lysine, cysteine, and tyrosine residues. DEPC-treated water is used in handling of RNA in the laboratory, to reduce the risk of RNA being degraded by RNases. Water is usually treated with 0.1% v/v diethylpyrocarbonate for at least 2 hours at 37 °C and then autoclaved to inactivate traces of DEPC. Inactivation of DEPC in this manner yields CO2, H2O and ethanol. Higher concentrations of DEPC are capable of deactivating larger amounts of RNase, but remaining traces or byproducts may inhibit further biochemical reactions such as in vitro translation. Furthermore, chemical modification of RNA such as carboxymethylation is possible when traces of DEPC or its byproducts are present, resulting in impaired recovery of intact RNA even after buffer exchange. DEPC is unstable in water and susceptible to hydrolysis to carbon dioxide and ethanol, especially in the presence of a nucleophile. For this reason, DEPC cannot be used with Tris or HEPES buffers. In contrast, it can be used with phosphate-buffered saline or MOPS. A handy rule is that enzymes or chemicals which have active -O:, -N: or -S: cannot be treated with DEPC to become RNase-free, as DEPC reacts with these species. Furthermore, DEPC degradation products can inhibit in vitro transcription.
Numerology
Chaldean Numerology
The numerical value of diethylpyrocarbonate in Chaldean Numerology is: 8
Pythagorean Numerology
The numerical value of diethylpyrocarbonate in Pythagorean Numerology is: 2
Translation
Find a translation for the diethylpyrocarbonate definition in other languages:
Select another language:
- - Select -
- 简体中文 (Chinese - Simplified)
- 繁體中文 (Chinese - Traditional)
- Español (Spanish)
- Esperanto (Esperanto)
- 日本語 (Japanese)
- Português (Portuguese)
- Deutsch (German)
- العربية (Arabic)
- Français (French)
- Русский (Russian)
- ಕನ್ನಡ (Kannada)
- 한국어 (Korean)
- עברית (Hebrew)
- Gaeilge (Irish)
- Українська (Ukrainian)
- اردو (Urdu)
- Magyar (Hungarian)
- मानक हिन्दी (Hindi)
- Indonesia (Indonesian)
- Italiano (Italian)
- தமிழ் (Tamil)
- Türkçe (Turkish)
- తెలుగు (Telugu)
- ภาษาไทย (Thai)
- Tiếng Việt (Vietnamese)
- Čeština (Czech)
- Polski (Polish)
- Bahasa Indonesia (Indonesian)
- Românește (Romanian)
- Nederlands (Dutch)
- Ελληνικά (Greek)
- Latinum (Latin)
- Svenska (Swedish)
- Dansk (Danish)
- Suomi (Finnish)
- فارسی (Persian)
- ייִדיש (Yiddish)
- հայերեն (Armenian)
- Norsk (Norwegian)
- English (English)
Word of the Day
Would you like us to send you a FREE new word definition delivered to your inbox daily?
Citation
Use the citation below to add this definition to your bibliography:
Style:MLAChicagoAPA
"diethylpyrocarbonate." Definitions.net. STANDS4 LLC, 2024. Web. 18 Apr. 2024. <https://www.definitions.net/definition/diethylpyrocarbonate>.
Discuss these diethylpyrocarbonate definitions with the community:
Report Comment
We're doing our best to make sure our content is useful, accurate and safe.
If by any chance you spot an inappropriate comment while navigating through our website please use this form to let us know, and we'll take care of it shortly.
Attachment
You need to be logged in to favorite.
Log In