What does comet assay mean?

Definitions for comet assay
comet as·say

Here are all the possible meanings and translations of the word comet assay.

Freebase

  1. Comet assay

    The Single Cell Gel Electrophoresis assay is an uncomplicated and sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell. It was first developed by Östling & Johansson in 1984 and later modified by Singh et al. in 1988. It has since increased in popularity as a standard technique for evaluation of DNA damage/repair, biomonitoring and genotoxicity testing. It involves the encapsulation of cells in a low-melting-point agarose suspension, lysis of the cells in neutral or alkaline conditions, and electrophoresis of the suspended lysed cells. The term "comet" refers to the pattern of DNA migration through the electrophoresis gel, which often resembles a comet. Principle: The comet assay is a simple method for measuring deoxyribonucleic acid strand breaks in eukaryotic cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled loops of DNA linked to the nuclear matrix. Electrophoresis at high pH results in structures resembling comets, observed by fluorescence microscopy; the intensity of the comet tail relative to the head reflects the number of DNA breaks. The likely basis for this is that loops containing a break lose their supercoiling and become free to extend toward the anode. This is followed by visual analysis with staining of DNA and calculating fluorescence to determine the extent of DNA damage. This can be performed by manual scoring or automatically by imaging software.

U.S. National Library of Medicine

  1. Comet Assay

    A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.

How to pronounce comet assay?

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How to say comet assay in sign language?

  1. cometassay

Numerology

  1. Chaldean Numerology

    The numerical value of comet assay in Chaldean Numerology is: 5

  2. Pythagorean Numerology

    The numerical value of comet assay in Pythagorean Numerology is: 4

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  1. comet assay


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"comet assay." Definitions.net. STANDS4 LLC, 2019. Web. 19 Jul 2019. <https://www.definitions.net/definition/comet+assay>.

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