What does spectrofluorometer mean?
Definitions for spectrofluorometer
spec·troflu·o·rom·e·ter
This dictionary definitions page includes all the possible meanings, example usage and translations of the word spectrofluorometer.
Wiktionary
spectrofluorometernoun
A spectrometer used in fluorescence spectroscopy
Wikipedia
Spectrofluorometer
A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample. A certain excitation wavelength is selected, and the emission is observed either at a single wavelength, or a scan is performed to record the intensity versus wavelength, also called an emission spectrum. The instrument is used in fluorescence spectroscopy.
Wikidata
Spectrofluorometer
The spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample. A certain excitation wavelength is selected, and the emission is observed either at a single wavelength or a scan is performed to record the intensity versus wavelength also called an emission spectra. See Fluorescence spectroscopy Generally spectrofluorometers use high intensity light sources to bombard a sample with as many photons as possible. This allows for the maximum number of molecules to be in the excited state at any one point in time. The light is either passed through a filter, selecting a fixed wavelength, or monochromator, which allows you to select a wavelength of interest to use as the exciting light. The emission is collected at 90 degrees to the exciting light. The emission too is either passed through a filter or a monochromator before being detected by a PMT, photodiode, or CCD detector. The signal can either be processed as a digital or analog output. Systems vary greatly and a few things must be considered when choosing. The first is signal to noise. There are many ways to look at the signal to noise of a given system but the accepted standard is by using water Raman. Sensitivity or detection limit is another spec to look at, that is how little light they can measure. The standard for this would be fluorescein in NaOH, typical values for a high end instrument are in the femtomolar range. Stray light is another big issue in these instruments. Stray light is basically how monochromatic the light is. This matters when you have a highly scattering sample, however one can always use an excitation wavelength further away from the emission band to negate this issue or use a laser or interference filter.
Numerology
Chaldean Numerology
The numerical value of spectrofluorometer in Chaldean Numerology is: 4
Pythagorean Numerology
The numerical value of spectrofluorometer in Pythagorean Numerology is: 1
Translations for spectrofluorometer
From our Multilingual Translation Dictionary
- 熒光分光光度計Chinese
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"spectrofluorometer." Definitions.net. STANDS4 LLC, 2024. Web. 18 Apr. 2024. <https://www.definitions.net/definition/spectrofluorometer>.
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