an assay that shows specific antigens in tissues by the use of markers that are either fluorescent dyes or enzymes (such as horseradish peroxidase)
The analytical process of finding proteins in cells of a tissue microtome section exploiting the principle of antibodies binding specifically to antigens in biological tissues.
Immunohistochemistry or IHC refers to the process of detecting antigens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. IHC takes its name from the roots "immuno," in reference to antibodies used in the procedure, and "histo," meaning tissue. Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. A video showing preparation for immunohistochemistry and then 20 minutes scanning through immunohistochemically stained tissues near colon cancers, shows different degrees of deficiency in three DNA repair proteins and one mitochondrial protein. Specific molecular markers are characteristic of particular cellular events such as proliferation or cell death. IHC is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue. Visualising an antibody-antigen interaction can be accomplished in a number of ways. In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyse a colour-producing reaction. Alternatively, the antibody can also be tagged to a fluorophore, such as fluorescein or rhodamine.
U.S. National Library of Medicine
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The numerical value of immunohistochemistry in Chaldean Numerology is: 3
The numerical value of immunohistochemistry in Pythagorean Numerology is: 6
Images & Illustrations of immunohistochemistry
Translations for immunohistochemistry
From our Multilingual Translation Dictionary
Get even more translations for immunohistochemistry »
Find a translation for the immunohistochemistry definition in other languages:
Select another language:
Discuss these immunohistochemistry definitions with the community:
Word of the Day
Would you like us to send you a FREE new word definition delivered to your inbox daily?
Use the citation below to add this definition to your bibliography:
"immunohistochemistry." Definitions.net. STANDS4 LLC, 2017. Web. 19 Oct. 2017. <http://www.definitions.net/definition/immunohistochemistry>.