Definitions for immunofluorescence
ˌɪm yə noʊ flʊˈrɛs əns, -flɔ-, -floʊ-, ɪˌmyu-im·munoflu·o·res·cence

Princeton's WordNetRate this definition:0.0 / 0 votes

1. immunofluorescencenoun

   (immunology) a technique that uses antibodies linked to a fluorescent dye in order to study antigens in a sample of tissue

WiktionaryRate this definition:0.0 / 0 votes

1. immunofluorescencenoun

   a technique that uses a fluorochrome to indicate a specific antigen-antibody reaction

WikipediaRate this definition:0.0 / 0 votes

1. Immunofluorescence

   Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets within a cell, and therefore allows visualization of the distribution of the target molecule through the sample. The specific region an antibody recognizes on an antigen is called an epitope. There have been efforts in epitope mapping since many antibodies can bind the same epitope and levels of binding between antibodies that recognize the same epitope can vary. Additionally, the binding of the fluorophore to the antibody itself cannot interfere with the immunological specificity of the antibody or the binding capacity of its antigen. Immunofluorescence is a widely used example of immunostaining (using antibodies to stain proteins) and is a specific example of immunohistochemistry (the use of the antibody-antigen relationship in tissues). This technique primarily makes use of fluorophores to visualise the location of the antibodies.Immunofluorescence can be used on tissue sections, cultured cell lines, or individual cells, and may be used to analyze the distribution of proteins, glycans, and small biological and non-biological molecules. This technique can even be used to visualize structures such as intermediate-sized filaments. If the topology of a cell membrane has yet to be determined, epitope insertion into proteins can be used in conjunction with immunofluorescence to determine structures. Immunofluorescence can also be used as a "semi-quantitative" method to gain insight into the levels and localization patterns of DNA methylation since it is a more time-consuming method than true quantitative methods and there is some subjectivity in the analysis of the levels of methylation. Immunofluorescence can be used in combination with other, non-antibody methods of fluorescent staining, for example, use of DAPI to label DNA. Several microscope designs can be used for analysis of immunofluorescence samples; the simplest is the epifluorescence microscope, and the confocal microscope is also widely used. Various super-resolution microscope designs that are capable of much higher resolution can also be used.

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1. immunofluorescence

   Immunofluorescence is a laboratory technique used in biological research for detecting a specific protein or antigen in cells or tissues. The method utilizes an antibody that can specifically bind to the protein of interest, and this antibody is coupled to a fluorescent dye. When exposed to certain types of light, the dye will fluoresce, indicating the presence and location of the protein. This technique can be used for studies in cell biology, microbiology, and molecular pathology.

WikidataRate this definition:0.0 / 0 votes

1. Immunofluorescence

   Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on microbiological samples. This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets within a cell, and therefore allows visualisation of the distribution of the target molecule through the sample. Immunofluorescence is a widely used example of immunostaining and is a specific example of immunohistochemistry that makes use of fluorophores to visualise the location of the antibodies. Immunofluorescence can be used on tissue sections, cultured cell lines, or individual cells, and may be used to analyse the distribution of proteins, glycans, and small biological and non-biological molecules. Immunofluoresence can be used in combination with other, non-antibody methods of fluorescent staining, for example, use of DAPI to label DNA. Several microscope designs can be used for analysis of immunofluorescence samples; the simplest is the epifluorescence microscope, and the confocal microscope is also widely used. Various super-resolution microscope designs that are capable of much higher resolution can also be used.

Matched Categories

1. • Immunology
   • Technique

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Numerology

1. Chaldean Numerology

   The numerical value of immunofluorescence in Chaldean Numerology is: 1

2. Pythagorean Numerology

   The numerical value of immunofluorescence in Pythagorean Numerology is: 4

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References

1. ^ Princeton's WordNet
   http://wordnetweb.princeton.edu/perl/webwn?s=immunofluorescence
2. ^ Wiktionary
   https://en.wiktionary.org/wiki/Immunofluorescence
3. ^ Wikipedia
   https://en.wikipedia.org/wiki/Immunofluorescence
4. ^ ChatGPT
   https://chat.openai.com
5. ^ Wikidata
   https://www.wikidata.org/w/index.php?search=immunofluorescence