Definitions for dideoxynucleotide
dideoxynu·cleotide

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1. dideoxynucleotidenoun

   Any nucleotide formed from a deoxynucleotide by loss of an a second hydroxy group from the deoxyribose group

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1. Dideoxynucleotide

   Dideoxynucleotides are chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. They are also known as 2',3' because both the 2' and 3' positions on the ribose lack hydroxyl groups, and are abbreviated as ddNTPs (ddGTP, ddATP, ddTTP and ddCTP).

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1. Dideoxynucleotide

   Dideoxynucleotides are chain-terminating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. They are also known as 2',3' dideoxynucleotides, and abbreviated as ddNTPs. The absence of the 3'-hydroxyl group means that, after being added by a DNA polymerase to a growing nucleotide chain, no further nucleotides can be added as no phosphodiester bond can be created based on the fact that deoxyribonucleoside triphosphates allow DNA chain synthesis to occur through a condensation reaction between the 5' phosphate of the current nucleotide with the 3' hydroxyl group of the previous nucleotide. The dideoxyribonucleotides do not have a 3' hydroxyl group, hence no further chain elongation can occur once this dideoxynucleotide is on the chain. This can lead to the termination of the DNA sequence. Thus, these molecules form the basis of the dideoxy chain-termination method of DNA sequencing, which was developed by Frederick Sanger in 1977. Dideoxynucleotides are useful in the sequencing of DNA in combination with electrophoresis. A DNA sample that undergoes PCR in a mixture containing all four deoxynucleotides and one dideoxynucleotide will produce strands of length equal to the position of each base of the type that complements the type having a dideoxynucleotide present. That is, each nucleotide base of that particular type has a probability of being bonded to not a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation. Thus, if the sample then undergoes electrophoresis, there will be a band present for each length at which the complement of the dideoxynucleotide is present. It is now common to use fluorescent dideoxynucleotides such that each one of the four has a different fluorescence that can be detected by a sequencer; thus only one reaction is needed.

How to pronounce dideoxynucleotide?

1. Alex

   US English

   David

   US English

   Mark

   US English

   Daniel

   British

   Libby

   British

   Mia

   British

   Karen

   Australian

   Hayley

   Australian

   Natasha

   Australian

   Veena

   Indian

   Priya

   Indian

   Neerja

   Indian

   Zira

   US English

   Oliver

   British

   Wendy

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   Fred

   US English

   Tessa

   South African

How to say dideoxynucleotide in sign language?

Numerology

1. Chaldean Numerology

   The numerical value of dideoxynucleotide in Chaldean Numerology is: 7

2. Pythagorean Numerology

   The numerical value of dideoxynucleotide in Pythagorean Numerology is: 5

References

1. ^ Wiktionary
   https://en.wiktionary.org/wiki/Dideoxynucleotide
2. ^ Wikipedia
   https://en.wikipedia.org/wiki/Dideoxynucleotide
3. ^ Wikidata
   https://www.wikidata.org/w/index.php?search=dideoxynucleotide